Buy Real-Time pcr thermal cycler detection system MSLPCR06 from Medsinglong Parameters:

Order No.:MSLPCR06A
Model:MSLPCR06A
Channel:2    
Reaction per run:96    
Block Format:96-well 0.2 ml    
Color combinations:Up to 2    
Light Source:High brightness monochrome LED    
Detector:High sensitive cold light CCD    
Detection dynamic range:10^2～10^10    
Sensitivity:Down to 1 copy    
Reaction volume:15ul -100ul    
Chemistry:all real-time PCR-based chmistries.Flexibility for chemistries with or without passive reference dye    
Excitation source:White LED    
Excitation filters/colors:Channel 1- 470nm; Channel 2- 525nm    
Detection filters/colors:Channel 1- 525nm; Channel 2- 570nm    
Kits & Reagent:Channel 1- FAM/SYBR; Channel 2- VIC/HEX/JOE/TET/TAMRA    
Block Material:Peltier    
Accuracy:≤±0.1℃    
Uniformity:±0.4℃ (10 sec after reaching 95℃); ±0.2℃ (10 sec after reaching 55℃)    
Temperature range :0-100℃    
Max ramp rate:6℃
Gradient range    :30-100℃    
PC operation system:Windows XP/VISTA/Windows7/Windows8    
MSLPCR06 operation system:Linus    
CPU:A8    
Communication:LAN/Wi-Fi    
Multiple control:support
Application Available :Gene Expression,Genotyping,Copy Number Variation,Protein Detection,MicroRNA,Pathogen Detection    
Dimension(L*W*H):592mm*440mm*280mm    
Package dimension(L*W*H):840mm*710mm*530mm    
Net Weight (Kg)    :28.5
Gross Weight (Kg):60    
Power Supply:
1.Standard configuration 220V/50HZ
2.Available upon special request 220V/60HZ,110V/60HZ,
3.Please note: Power supply is defaulted as standard configuration.Please remark on special request.Shipment date for special request might be later than standard configuration, please contact our salesperson to confirm.    
Buy Real-Time pcr thermal cycler detection system MSLPCR06 from Medsinglong Notice

Order No.:MSLPCR06B

Buy Real-Time pcr thermal cycler detection system MSLPCR06 from Medsinglong
Model:MSLPCR06B
Channel:5    
Reaction per run:96    
Block Format:96-well 0.2 ml    
Color combinations:Up to 5    
Light Source:High brightness monochrome LED    
Detector:High sensitive cold light CCD    
Detection dynamic range:10^2～10^10    
Sensitivity:Down to 1 copy    
Reaction volume:15ul -100ul    
Chemistry:all real-time PCR-based chmistries. Flexibility for chemistries with or without passive reference dye    
Excitation source:White LED    
Excitation filters/colors:Channel 1- 470nm; Channel 2- 525nm; Channel 3- 585nm; Channel 4- 610nm    
Detection filters/colors:Channel 1- 525nm; Channel 2- 570nm; Channel 3- 610nm; Channel 4- 678nm    
Kits & Reagent:Channel 1- FAM/SYBR; Channel 2- VIC/HEX/JOE/TET/TAMRA; Channel 3- ROX/TEXRAD; Channel 4- CY5    
Block Material:Peltier    
Accuracy:≤±0.1℃    
Uniformity:±0.4℃ (10 sec after reaching 95℃);±0.2℃ (10 sec after reaching 55℃)    
Temperature range :0-100℃    
Max ramp rate:6℃    
Gradient range:30-100℃    
PC operation system:Windows XP/VISTA/Windows7/Windows8    
MSLPCR06 operation system:Linus    
CPU:A8    
Communication:LAN/Wi-Fi    
Multiple control:Support    
Application Available :Gene Expression,Genotyping,Copy Number Variation,Protein Detection,MicroRNA,Pathogen Detection    
Dimension(L*W*H):592mm*440mm*280mm    
Package dimension(L*W*H):840mm*710mm*530mm    
Net Weight (Kg):28.7    
Gross Weight (Kg):60    
Power Supply:
1.Standard configuration 220V/50HZ    
2.Available upon special request  220V/60HZ, 110V/60HZ    
3.Please note: Power supply is defaulted as standard configuration. Please remark on special request. Shipment date for special request might be later than standard configuration, please contact our salesperson to confirm.    
Notice

Buy Real-Time pcr thermal cycler detection system MSLPCR06 from Medsinglong

Which real-time or digital PCR system is right for you?

To understand why traditional PCR is limited, it is important to understand what happens during the PCR reaction. A basic PCR run can be divided into three phases:

Index period

Accumulated product exactly doubles with each cycle (assuming 100% reaction efficiency). The reaction is highly specific and precise. Exponential amplification occurs because all reagents are freshly available and the kinetics of the reaction drive the reaction in favor of amplicon doubling.

Linear phase (high variability)
As the reaction continues, some reagents are consumed by amplification. The reaction starts to slow down, and the PCR product no longer doubles with each cycle.

Plateau (endpoint: gel detection using conventional methods)
The reaction stops, no more product is produced, and if left long enough, the PCR product will start to degrade. Because each sample has different reaction kinetics, each tube or reaction will plateau at a different time point. These differences can be seen during the plateau. During the plateau period, conventional PCR performs the measurement, also known as endpoint detection.

Variation in plateau measurements with conventional PCR
Three replicate samples had the same amount of DNA at the start of the reaction and different amounts of PCR product during the plateau phase of the reaction (due to changes in reaction kinetics). Therefore, it will be more accurate to make measurements during the exponential period, where replicate samples expand exponentially.

Real-time PCR measures during exponential phase for more accurate quantitation
Real-time PCR focuses on the exponential phase because it provides more precise data for quantification. During the exponential period, the real-time PCR instrument calculates two values. The threshold line is the level of detection at which the fluorescence intensity of the reaction exceeds background. The PCR cycle at which the sample reaches this level is called the cycle threshold (Ct). Ct values are used for downstream quantification or presence/absence detection. By comparing the Ct values of a sample of unknown concentration with a series of standards, the amount of template DNA in an unknown reaction can be accurately determined.

Digital PCR counts individual molecules for absolute quantification
Digital PCR works by splitting the sample into many individual real-time PCR reactions; some of these reactions contain the molecule of interest (positive) and others do not (negative). After PCR analysis, a fraction of the negative results are used to generate absolute results for the exact amount of target molecule in the sample without the need for reference standards or endogenous controls.

MedsinglongPCR system, from kits, instruments, enzymes, master mixes, reagents, optical cartridges, plastic accessories, spare parts to software etc. Medsinglong real-time quantitative PCR system is a fully integrated quantitative PCR amplification, detection and data analysis system. Medsinglong increases productivity with its unique modular and flexible design, intuitive touch screen interface, advanced and easy-to-use reporting system, and monitors more than 120 attributes through built-in online diagnostic system, so that when analytical problems or instrument problems arise find out the reason.