Purchase Real-Time PCR System pcr thermo cycler MSLPCR16E from Medsinglong Thermal Block:

Sample capacity(wells):    48        
Reaction Volume    :5-100 μL         
Consumables: 0.2ml 8-strip tubes ,0.2ml PCR single tube(Optical flat cap,clear tube)    
Temperature range  :0°C-100°C        
Heating/cooling method:Peltier        
Max heating rate :8.0°C/sec        
Average heating rate :6.0°C/sec        
Max cooling rate :5.5°C/sec        
Average cooling rate :4.0°C/sec        
Temperature accuracy :±0.1℃        
Temperature uniformity:±0.1℃        
Gradient Rang:1°C-40°C
Gradient block:8 row
Special temperature portocol:Gradient PCR,Long PCR,Touch Down PCR

Purchase Real-Time PCR System pcr thermo cycler MSLPCR16E from Medsinglong

Heat Lid:
Temperature range:Room Temperature–110°C

Optical System:        
Excitation Source:4LEDs    
Detector:Photodiode        
Detection Position:Excitation and scan from lateral        
Detection method:4 channels scanning at the same time, no edge effect.        
Detection time:2 seconds for 48 wells for all channels    

Range of excitation/ emission wavelengths (nm):  

 Purchase Real-Time PCR System pcr thermo cycler MSLPCR16E from Medsinglong
1  465 /510 (FAM,SYBR Green I，SYTO9，EvaGreen，LC Green)

2  527/ 563 (HEX,VIC,TET,JOE)

3  580 /616 (ROX,Texas Red)

4  632/ 664( Cy5)

Probe:Taqman Probe,Molecular beacons probe，Scorpion probe        
Multiplexing:Up to 4 targets    
Fluorescence Linearity:r≥0.990        
Fluorescence Dynamic Range:Adjustable

Performance:        
Sample Linearity:/r/≥0.999        
Sample repeatability:Ct value CV≤0.5%        
Sample Dynamic Range:1-1010 copies        

Purchase Real-Time PCR System pcr thermo cycler MSLPCR16E from Medsinglong Function:

Data Analysis modes:
Qualitative analysis,Absolute quantification,Relative,quantification,Genotyping analysis,Endpoint analysis，Melt curve analysis, High Resolution Melting"        
Control modes:
1.7 touch screen 

2.PC direct control

3.WLAN control(One PC can control max 10 units, and device can be controled by any PC in the WLAN)"        
Data Stroage :Upload and download through USB disk,1000 results can be stored  in machine        
Power failure protection:Automaticly start running experiments after power supply, no need wait PC software        
Customize Report:Templetes reserved ,report can customized        
Administration Management:Administrator can set funtions limts for users        
Fault Management:Fault report and analysis, solution instruction        
LIS connection    CSV,Excel,TXT format data output,open port for LIS connection        

Purchase Real-Time PCR System pcr thermo cycler MSLPCR16E from Medsinglong Others:
PC operating system:Win 7, Win 10        
Communication Port:1 ethernet and 2  USB        
Footprint(WxDxH):260mm X 400mm X 2605mm        
Weight:11kg        
Power usage:AC 100 to 125 V / 200 to 240 V (50/60 Hz).        
power consumption:600VA        
Work environment:Tempereture:10°C~30°C  Humidity:20%~85%

Which real-time or digital PCR system is right for you?

To understand why traditional PCR is limited, it is important to understand what happens during the PCR reaction. A basic PCR run can be divided into three phases:

Index period

Accumulated product exactly doubles with each cycle (assuming 100% reaction efficiency). The reaction is highly specific and precise. Exponential amplification occurs because all reagents are freshly available and the kinetics of the reaction drive the reaction in favor of amplicon doubling.

Linear phase (high variability)
As the reaction continues, some reagents are consumed by amplification. The reaction starts to slow down, and the PCR product no longer doubles with each cycle.

Plateau (endpoint: gel detection using conventional methods)
The reaction stops, no more product is produced, and if left long enough, the PCR product will start to degrade. Because each sample has different reaction kinetics, each tube or reaction will plateau at a different time point. These differences can be seen during the plateau. During the plateau period, conventional PCR performs the measurement, also known as endpoint detection.

Variation in plateau measurements with conventional PCR
Three replicate samples had the same amount of DNA at the start of the reaction and different amounts of PCR product during the plateau phase of the reaction (due to changes in reaction kinetics). Therefore, it will be more accurate to make measurements during the exponential period, where replicate samples expand exponentially.

Real-time PCR measures during exponential phase for more accurate quantitation
Real-time PCR focuses on the exponential phase because it provides more precise data for quantification. During the exponential period, the real-time PCR instrument calculates two values. The threshold line is the level of detection at which the fluorescence intensity of the reaction exceeds background. The PCR cycle at which the sample reaches this level is called the cycle threshold (Ct). Ct values are used for downstream quantification or presence/absence detection. By comparing the Ct values of a sample of unknown concentration with a series of standards, the amount of template DNA in an unknown reaction can be accurately determined.

Digital PCR counts individual molecules for absolute quantification
Digital PCR works by splitting the sample into many individual real-time PCR reactions; some of these reactions contain the molecule of interest (positive) and others do not (negative). After PCR analysis, a fraction of the negative results are used to generate absolute results for the exact amount of target molecule in the sample without the need for reference standards or endogenous controls.

MedsinglongPCR system, from kits, instruments, enzymes, master mixes, reagents, optical cartridges, plastic accessories, spare parts to software etc. Medsinglong real-time quantitative PCR system is a fully integrated quantitative PCR amplification, detection and data analysis system. Medsinglong increases productivity with its unique modular and flexible design, intuitive touch screen interface, advanced and easy-to-use reporting system, and monitors more than 120 attributes through built-in online diagnostic system, so that when analytical problems or instrument problems arise find out the reason.