Detection system Real-Time PCR machine MSLPCR05 description

MSLPCR05 has a faster heating and cooling rate, better temperature control accuracy,temperature uniformity and the machine stability
with the specially-made Peltier by Ferrotec,advanced optical fiber technology, the new wide voltage power supply, its unique block radiating
patent technology and the bottom detection patent mode. It can be used with Windows Tablet PC. This new touch screen operation will bring you
the ultimate experience of human-computer interaction. The new and innovative LineGene K Plus Real-time PCR detection system can adapt to
different levels of customer requirements, which can fully meets various requirements of scientific research and clinical applications.

Genetic Analysis Detection system Real-Time PCR machine MSLPCR05 thermal cycler price

Detection system PCR machine MSLPCR05 feature

Advanced thermoelectric refrigeration technology and super fast heat cycle system ensure fast
and stable heating and cooling.

Multipoint temperature control ensures better temperature uniformity for 48 sample holes.

SOAK ensures low temperature conservation of PCR kits.

Hot lid realizes PCR il-free operation.

Auto hot lid, no need for manual open/close, ensures constant pressure regarding various heights'
PCR tubes.

Which real-time or digital PCR system is right for you?

To understand why traditional PCR is limited, it is important to understand what happens during the PCR reaction. A basic PCR run can be divided into three phases:

Index period

Accumulated product exactly doubles with each cycle (assuming 100% reaction efficiency). The reaction is highly specific and precise. Exponential amplification occurs because all reagents are freshly available and the kinetics of the reaction drive the reaction in favor of amplicon doubling.

Linear phase (high variability)
As the reaction continues, some reagents are consumed by amplification. The reaction starts to slow down, and the PCR product no longer doubles with each cycle.

Plateau (endpoint: gel detection using conventional methods)
The reaction stops, no more product is produced, and if left long enough, the PCR product will start to degrade. Because each sample has different reaction kinetics, each tube or reaction will plateau at a different time point. These differences can be seen during the plateau. During the plateau period, conventional PCR performs the measurement, also known as endpoint detection.

Variation in plateau measurements with conventional PCR
Three replicate samples had the same amount of DNA at the start of the reaction and different amounts of PCR product during the plateau phase of the reaction (due to changes in reaction kinetics). Therefore, it will be more accurate to make measurements during the exponential period, where replicate samples expand exponentially.

Real-time PCR measures during exponential phase for more accurate quantitation
Real-time PCR focuses on the exponential phase because it provides more precise data for quantification. During the exponential period, the real-time PCR instrument calculates two values. The threshold line is the level of detection at which the fluorescence intensity of the reaction exceeds background. The PCR cycle at which the sample reaches this level is called the cycle threshold (Ct). Ct values are used for downstream quantification or presence/absence detection. By comparing the Ct values of a sample of unknown concentration with a series of standards, the amount of template DNA in an unknown reaction can be accurately determined.

Digital PCR counts individual molecules for absolute quantification
Digital PCR works by splitting the sample into many individual real-time PCR reactions; some of these reactions contain the molecule of interest (positive) and others do not (negative). After PCR analysis, a fraction of the negative results are used to generate absolute results for the exact amount of target molecule in the sample without the need for reference standards or endogenous controls.

MedsinglongPCR system, from kits, instruments, enzymes, master mixes, reagents, optical cartridges, plastic accessories, spare parts to software etc. Medsinglong real-time quantitative PCR system is a fully integrated quantitative PCR amplification, detection and data analysis system. Medsinglong increases productivity with its unique modular and flexible design, intuitive touch screen interface, advanced and easy-to-use reporting system, and monitors more than 120 attributes through built-in online diagnostic system, so that when analytical problems or instrument problems arise find out the reason.